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KMID : 0545120150250040452
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 4 p.452 ~ p.458
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Whole Cell Bioconversion of Ricinoleic Acid to 12-Ketooleic Acid by Recombinant Corynebacterium glutamicum-Based Biocatalyst
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Lee Byeong-Hun
Lee Sae-Bom
Kim Hyeon-Soo
Jeong Ki-Jun
Park Jin-Byung
Park Kyung-Moon
Lee Jin-Won
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Abstract
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The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35°C, and 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; and 3.9 mM 12- ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicumbased biocatalyst.
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KEYWORD
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12-Ketooleic acid, Ricinoleic acid, Secondary alcohol dehydrogenase, Whole cell bioconversion, Corynebacterium glutamicum
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